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[KO]Phospho-4E BP1 (Thr46) Rabbit mAb

Western blot analysis of Phospho-4E BP1 (Thr46) in HeLa(WT) and Phospho-4E BP1 (Thr46)-Knockout HeLa(KO) using Phospho-4E BP1 (Thr46) antibody.
Western blot analysis of Phospho-eIF4EBP1 (Thr46) in K562, C6, 3T3, Hela lysates using Phospho-4E BP1 (Thr46) antibody.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using eIF4EBP1/eIF4EBP2/eIF4EBP3 (Phospho- T45) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P
Primary Accession	Q13541
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Purification	Affinity Purified
Calculated MW	12580 Da

Additional Information
Gene ID	1978
Other Names	EIF4EBP1
Dilution	WB~~1:1000-1:4000 IHC-P~~1:50~200
Format	1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	EIF4EBP1
Function	Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Cellular Location	Cytoplasm. Nucleus. Note=Localization to the nucleus is unaffected by phosphorylation status. {ECO:0000250\|UniProtKB:Q60876}

Research Areas

一抗

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.

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Cat# AP74966

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[KO]Phospho-4E BP1 (Thr46) Rabbit mAb

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